Galaxy Training: Using Galaxy for RNA-seq
This practical aims to familiarize you with the Galaxy user interface & execute a complete analysis of RNA-seq data.
It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. In this tutorial, we will introduce you to bare basics of Galaxy:
- Getting data from external databases such as UCSC
- Performing simple data manipulation
- Understanding Galaxy’s History system
- Creating and running a workflow
The RNA-seq data analysis part comprises:
- Filtering and quality control of reads
- Mapping of reads to reference genomes
- Examples of downstream data-analysis possibilities (variant calling, differential expression, etc.)